Harvard Forest Data Archive

HF348

White Pine Girdling and Compression Experiment at Harvard Forest 2017-2019

Related Publications

Data

• hf348-01: allometric data (preview)
• hf348-02: circumferential pressure distribution (preview)
• hf348-03: nonstructural carbon data - leaves (preview)
• hf348-04: nonstructural carbon data - 1st cm of xylem (preview)
• hf348-05: nonstructural carbon data - re-runs for quality assurance (preview)
• hf348-06: nonstructural carbon data - 2nd cm of xylem (preview)
• hf348-07: nonstructural carbon data - roots (preview)
• hf348-08: nonstructural carbon data - roots re-runs (preview)
• hf348-09: pressure data (preview)
• hf348-10: respiration data (preview)
• hf348-11: water potential data - needles (preview)
• hf348-12: water potential data - branches (preview)
• hf348-13: wood anatomy (preview)
• hf348-14: R code density anomaly analysis

Overview

• Lead: Tim Rademacher, Andrew Richardson
• Investigators: Mark VanScoy
• Contact: Information Manager
• Start date: 2017
• End date: 2019
• Status: complete
• Location: Tom Swamp Tract (Harvard Forest)
• Latitude: 42.51 degrees
• Longitude: -72.22 degrees
• Elevation: 340 meter
• Datum: WGS84
• Taxa: Pinus strobus
• Release date: 2023
• Language: English
• EML file: knb-lter-hfr.348.4
• DOI: digital object identifier
• EDI: data package
• DataONE: data package
• Related links:
• Density Anomalies in White Pine at Harvard Forest 2019
• Investigating Wood Growth, Wood Properties and Carbon Metabolism in Young White Pines in Tom Swamp
• Study type: short-term measurement
• Research topic: forest-atmosphere exchange; large experiments and permanent plot studies; physiological ecology, population dynamics and species interactions
• LTER core area: primary production
• Keywords: allometry, biomass, carbohydrates, carbon, pine, respiration, tree physiology, wood
• Abstract:

  Wood formation is a crucial process for carbon sequestration on land, yet how variations in phloem-transported carbon affect wood formation, respiration and nonstructural carbon pools remains poorly understood.

  To better understand the role of carbon supply on allocation to wood formation, we constrained phloem transport using girdling and compression around the stem of 40 mature white pines to monitor the effects of contrasting carbon supply (enriched above and reduced below the manipulations) on local wood formation and respiration, as well as on nonstructural carbon pools in needles, stems, and roots.

  This data set contains all data measured during the experiment. This includes wood anatomy, xylogenesis, stem respiration, nonstructural carbon measurements in coarse roots, stems and needles, as well as pre-dawn water potential measurements and pressure measurements from underneath the phloem compression collars. Furthermore, we provide allometric measurements for all trees. The code to process these data and reproduce our results is available in hf348-14-density-anomaly-analysis-code.zip. For more details pertaining to the methods see Rademacher et al. (2021).

• Methods:

  All trees were monitored included a four-time characterisation of tree-ring formation and nonstructural carbon concentrations in needle, stem and root tissues, and a weekly survey of stem CO2 efflux. Additionally, tree water status was measured weekly.

  Wood formation and anatomy

  Tree-ring growth was characterised from stem microcores collected at several stem heights with a Trephor (Rossi et al., 2006) just before the experiment (July 3rd), twice during the experiment (August 8th and October 10th), and in late autumn (November 3rd). Ring widths were measured using the self-developed Tree Ring Image Analysis and Database platform (http://phenocam.nau.edu/TRIAD/) on microsection images captured using a digital slide-scanner (Zeiss Axio Scan.Z1, Germany) with a resolution of roughly 1.5 pixels per micrometer. Tracheid anatomical characteristics (e.g., averages of lumen width and cell-wall area over 20 micrometer-wide tangential bands) were obtained using ROXAS 3.0.285 (von Ar xand Carrer, 2014) from the November microsections.

  Stem respiration

  Starting one week before the beginning of the treatments (June 29th), an infrared gas analyser (LI-820, LI-COR, Lincoln, Nebraska, USA) with a circulating pump was attached to stem respiration chambers using a lid with two ports to constantly circulate air through the closed system (Carbone et al., 2019). The chamber CO2 concentration was measured at 1 Hz for at least one minute. The raw stem CO2 efflux and uncertainties were estimated using the RespChamberProc package (http://r-forge.r-project.org/projects/respchamberproc/) as developed by Perez-Priego et al. (2015).

  Nonstructural carbon

  Soluble sugar and starch concentrations in coarse roots, stems and needles were determined from tissue samples collected at the same time as the microcores. Coarse roots (at least 20 cm below the root collar) and stems were cored using a 5.15 mm increment borer (Haglӧf Company Group, Långsele, Sweden). Foliage samples were collected from a sun-exposed part of the crown with a pole pruner. About 40 mg of finely ground and dried powder for all tissue, tree and sampling date combinations was analysed following the protocol by Furze et al. (2018). Each batch of samples included on average 35 samples, at least 10 blanks - both tube and sample blanks - and between 9 and 12 laboratory control standards (red oak stem wood, Harvard Forest, Petersham, Massachusetts, USA; potato starch, Sigma Chemicals, St. Louis, Missouri, USA). We repeated extractions for batches that showed substantial deviations in the laboratory control standards (e.g., starch recovery fraction lower than 85%). Absorbance values for all samples were converted to concentrations in % dry weight and uncertainties with the self-developed R package NSCprocessR (https://github.com/TTRademacher/NSCprocessR).

  Water potential

  Pre-dawn needle and branch water potential were measured with a pressure chamber (Model 600, PMS Instruments, Albany, Oregon, USA) once per week per tree from the end of June to the beginning of November 2017 and twice in the two following years.

  For each measurement, two branch tips per tree were cut, sealed in air-tight plastic bags for transport. Initial tests showed that water potential could be determined accurately up to 20 minutes after cutting the tissue. Within 20 minutes of harvest, water potential was determined after re-cutting the tissue. Observed needle and branch water potential did not differ among treatments during the duration of the experiment or in the remaining growing season.

  For more details pertaining to the methods see Rademacher et al. (2021).

• Organization: Harvard Forest. 324 North Main Street, Petersham, MA 01366, USA. Phone (978) 724-3302. Fax (978) 724-3595.

• Project: The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts. (ROR).

• Funding: National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210.

• Use: This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.

• License: Creative Commons Zero v1.0 Universal (CC0-1.0)

• Citation: Rademacher T, Richardson A. 2023. White Pine Girdling and Compression Experiment at Harvard Forest 2017-2019. Harvard Forest Data Archive: HF348 (v.4). Environmental Data Initiative: https://doi.org/10.6073/pasta/af6457b0c5b9189b44fff4301a45f58a.

Detailed Metadata

hf348-01: allometric data

1. tree: tree identifier
2. treatment: treatment group
   • 1: control
   • 2: girdled
   • 3: compressed
   • 4: double compressed
3. stem.colour: colour of the stem from 1 for completely brown to 5 for completely green
4. measurement.heights: number of measurement heights on this stem (unit: number / missing value: NA)
5. d250: diameter at 250 cm above the ground (unit: centimeter / missing value: NA)
6. d200: diameter at 200 cm above the ground (unit: centimeter / missing value: NA)
7. d150: diameter at 150 cm above the ground (unit: centimeter / missing value: NA)
8. d100: diameter at 100 cm above the ground (unit: centimeter / missing value: NA)
9. d50: diameter at 50 cm above the ground (unit: centimeter / missing value: NA)
10. cambial.birth.year: year of formation of the oldest ring in the increment core from breast height
11. tree.height.2017: height of the tree as measured with a hypsometer in meters (unit: meter / missing value: NA)

hf348-02: circumferential pressure distribution

1. angle: angle from the ratchet in degree where measurement was performed (unit: degree / missing value: NA)
2. pressure: pressure exerted by the pressure collar (unit: megapascal / missing value: NA)
3. pressure.180: pressure exerted at the same angle as in “angle” by a collar offset by 180 degrees (unit: megapascal / missing value: NA)
4. max.pressure: maximum of pressure in the second and third columns (unit: megapascal / missing value: NA)

hf348-03: nonstructural carbon data - leaves

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-04: nonstructural carbon data - 1st cm of xylem

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-05: nonstructural carbon data - re-runs for quality assurance

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-06: nonstructural carbon data - 2nd cm of xylem

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-07: nonstructural carbon data - roots

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-08: nonstructural carbon data - roots re-runs

1. rc.labnumber: internal identification number for the Richardson and Carbone Lab
2. sample.id: sample identifier
3. tissue: tissue type, such as wood, leaf or roots
4. batch.id: batch identifier
5. samplelocation: location where the sample was collected
6. dateofsamplecollection: date the sample was collected
7. dateofsugaranalysis: date the sugar extraction and analysis was done
8. dateofstarchanalysis: date the starch extraction and analysis was done
9. massofemptytube: mass of empty tube in grams (unit: gram / missing value: NA)
10. massoftubeandsample: mass of tube with the sample in grams (unit: gram / missing value: NA)
11. absorbance490.1: first absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
12. absorbance490.2: second absorbance value read at 490 nm (unit: dimensionless / missing value: NA)
13. absorbance490.blank: absorbance value at 490 nm for the blank (unit: dimensionless / missing value: NA)
14. absorbance525.1: first absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
15. absorbance525.2: second absorbance value read at 525 nm (unit: dimensionless / missing value: NA)
16. dilutionfactorsugar: factor by which the sugar extract was diluted (unit: dimensionless / missing value: NA)
17. volumesugar: volume of the sugar extract (unit: milliliter / missing value: NA)
18. dilutionfactorstarch: factor by which the starch extract was diluted (unit: dimensionless / missing value: NA)
19. volumestarch: volume of the starch extract (unit: milliliter / missing value: NA)
20. comments: comments

hf348-09: pressure data

1. tree: tree identifier
2. treatment: treatment for this tree
   • compression: compression
   • double compression: double compression
3. collar: collar description
   • lower: at 1 m
   • middle: at 1.5 m
   • upper: at 2 m
4. sensor.number: whether it was the first or second sensor below the collar
   • 1: first sensor
   • 2: second sensor
5. p.2017.07.04: measured pressure on this date (unit: megapascal / missing value: NA)
6. p.2017.07.05: measured pressure on this date (unit: megapascal / missing value: NA)
7. p.2017.07.19: measured pressure on this date (unit: megapascal / missing value: NA)
8. p.2017.07.26: measured pressure on this date (unit: megapascal / missing value: NA)
9. p.2017.08.03: measured pressure on this date (unit: megapascal / missing value: NA)
10. p.2017.08.10: measured pressure on this date (unit: megapascal / missing value: NA)
11. p.2017.08.13.1: first measured pressure on this date (unit: megapascal / missing value: NA)
12. p.2017.08.13.2: second measured pressure on this date (unit: megapascal / missing value: NA)
13. p.2017.08.16: measured pressure on this date (unit: megapascal / missing value: NA)
14. p.2017.08.22: measured pressure on this date (unit: megapascal / missing value: NA)
15. p.2017.08.30: measured pressure on this date (unit: megapascal / missing value: NA)
16. p.2017.09.06: measured pressure on this date (unit: megapascal / missing value: NA)
17. p.2017.09.13: measured pressure on this date (unit: megapascal / missing value: NA)
18. p.2017.09.20: measured pressure on this date (unit: megapascal / missing value: NA)
19. p.2017.09.27: measured pressure on this date (unit: megapascal / missing value: NA)
20. p.2017.10.04: measured pressure on this date (unit: megapascal / missing value: NA)
21. p.2017.10.10: measured pressure on this date (unit: megapascal / missing value: NA)

hf348-10: respiration data

1. file: name of the raw data file
2. study: name of the study
3. treatment: treatment group
   • 1: control
   • 2: girdled
   • 3: compressed
   • 4: double compressed
4. tree: tree identifier
5. species: tree species
6. chamber: chamber identifier
   • 1: 0.5m above the ground
   • 2: 1.5m above the ground
   • 3: 2.5m above the ground
7. chamber.volume: volume of the respiration chamber (unit: cubicMeter / missing value: NA)
8. chamber.area: stem surface area covered by the chamber (unit: squareMeter / missing value: NA)
9. datetime: time stamp of when the measurement started
10. session: identifier for the session of respiration measurements
11. flux.raw: mean respiratory flux from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
12. sd.flux.raw: standard deviation of the respiratory flux from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
13. aic.raw: Akaike Information Criterion of model fitted to respiration curve after correction for water vapour dilution using atmospheric humidity (unit: dimensionless / missing value: NA)
14. r2.raw: R2 of fit to the respiration curve after correction for water vapour dilution using internal humidity (unit: dimensionless / missing value: NA)
15. flux.atm: mean respiratory flux after correction for water vapour dilution using atmospheric humidity from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
16. sd.flux.atm: standard deviation of the respiratory flux after correction for water vapour dilution using atmospheric humidity from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
17. aic.atm: Akaike Information Criterion of model fitted to respiration curve after correction for water vapour dilution using atmospheric humidity (unit: dimensionless / missing value: NA)
18. r2.atm: R2 of fit to the respiration curve after correction for water vapour dilution using atmospheric humidity (unit: dimensionless / missing value: NA)
19. flux.int: Mean respiratory flux after correction for water vapour dilution using internal humidity from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
20. sd.flux.int: standard deviation of the respiratory flux after correction for water vapour dilution using internal humidity from this chamber (unit: micromolePerMeterSquaredPerSecond / missing value: NA)
21. aic.int: Akaike Information Criterion of model fitted to respiration curve after correction for water vapour dilution using internal humidity (unit: dimensionless / missing value: NA)
22. r2.int: R2 of fit to the respiration curve after correction for water vapour dilution using internal humidity (unit: dimensionless / missing value: NA)
23. ea.pa: atmospheric pressure from the Fisher meteorological station (unit: pascal / missing value: NA)
24. airt.c: surface air temperature from the Fisher meteorological station (unit: celsius / missing value: NA)
25. soilt1.c: soil temperature at 2.25 cm near the Barn Tower (unit: celsius / missing value: NA)
26. soilt2.c: soil temperature at 6.8 cm near the Barn Tower (unit: celsius / missing value: NA)
27. soilt3.c: soil temperature at 12.85 cm near the Barn Tower (unit: celsius / missing value: NA)
28. soilt4.c: soil temperature at 22.75 cm near the Barn Tower (unit: celsius / missing value: NA)
29. pres.pa: atmospheric barometric pressure from the Fisher Meteorological station (unit: pascal / missing value: NA)
30. h2o.ppt.atm: atmospheric humidity from the Fisher meteorological station (parts per thousand) (unit: dimensionless / missing value: NA)
31. h2o.ppt.int: internal atmospheric humidity from the Li-Cor 840 (parts per thousand) (unit: dimensionless / missing value: NA)
32. vwc.daily: daily mean volumetric water content from probes at the Barn Tower (percent) (unit: dimensionless / missing value: NA)
33. vwc1: 10-minute mean volumetric water content at 2.25 cm near the Barn Tower (percent) (unit: dimensionless / missing value: NA)
34. vwc2: 10-minute mean volumetric water content at 6.8 cm near the Barn Tower (percent) (unit: dimensionless / missing value: NA)
35. vwc3: 10-minute mean volumetric water content at 12.85 cm near the Barn Tower (percent) (unit: dimensionless / missing value: NA)
36. vwc4: 10-minute mean volumetric water content at 22.75 cm near the Barn Tower (percent) (unit: dimensionless / missing value: NA)
37. total.rad: total downwelling shortwave radiation at top of Barn Tower (unit: wattPerMeterSquared / missing value: NA)
38. diffuse.rad: diffuse downwelling shortwave radiation at top of Barn Tower (unit: wattPerMeterSquared / missing value: NA)

hf348-11: water potential data - needles

1. tree.id: tree identifier
2. treatment: treatment group
   • 1: control
   • 2: girdled
   • 3: compressed
   • 4: double compressed
3. week.0: water potential, first measurement (unit: megapascal / missing value: NA)
4. week.1: water potential 1 week since first measurement (unit: megapascal / missing value: NA)
5. week.2: water potential 2 weeks since first measurement (unit: megapascal / missing value: NA)
6. week.3: water potential 3 weeks since first measurement (unit: megapascal / missing value: NA)
7. week.4: water potential 4 weeks since first measurement (unit: megapascal / missing value: NA)
8. week.5: water potential 5 weeks since first measurement (unit: megapascal / missing value: NA)
9. week.6: water potential 6 weeks since first measurement (unit: megapascal / missing value: NA)
10. week.7: water potential 7 weeks since first measurement (unit: megapascal / missing value: NA)
11. week.8: water potential 8 weeks since first measurement (unit: megapascal / missing value: NA)
12. week.9: water potential 9 weeks since first measurement (unit: megapascal / missing value: NA)
13. week.10: water potential 10 weeks since first measurement (unit: megapascal / missing value: NA)
14. week.11: water potential 11 weeks since first measurement (unit: megapascal / missing value: NA)
15. week.12: water potential 12 weeks since first measurement (unit: megapascal / missing value: NA)
16. week.13: water potential 13 weeks since first measurement (unit: megapascal / missing value: NA)
17. week.15: water potential 15 weeks since first measurement (unit: megapascal / missing value: NA)
18. week.17: water potential 17 weeks since first measurement (unit: megapascal / missing value: NA)
19. week.47: water potential 47 weeks since first measurement (unit: megapascal / missing value: NA)
20. week.53: water potential 53 weeks since first measurement (unit: megapascal / missing value: NA)
21. week.94: water potential 94 weeks since first measurement (unit: megapascal / missing value: NA)
22. week.105: water potential 105 weeks since first measurement (unit: megapascal / missing value: NA)

hf348-12: water potential data - branches

1. tree.id: tree identifier
2. treatment: treatment group
   • 1: control
   • 2: girdled
   • 3: compressed
   • 4: double compressed
3. week.0: water potential, first measurement (unit: megapascal / missing value: NA)
4. week.1: water potential 1 week since first measurement (unit: megapascal / missing value: NA)
5. week.2: water potential 2 weeks since first measurement (unit: megapascal / missing value: NA)
6. week.3: water potential 3 weeks since first measurement (unit: megapascal / missing value: NA)
7. week.4: water potential 4 weeks since first measurement (unit: megapascal / missing value: NA)
8. week.5: water potential 5 weeks since first measurement (unit: megapascal / missing value: NA)
9. week.6: water potential 6 weeks since first measurement (unit: megapascal / missing value: NA)
10. week.7: water potential 7 weeks since first measurement (unit: megapascal / missing value: NA)
11. week.8: water potential 8 weeks since first measurement (unit: megapascal / missing value: NA)
12. week.9: water potential 9 weeks since first measurement (unit: megapascal / missing value: NA)
13. week.10: water potential 10 weeks since first measurement (unit: megapascal / missing value: NA)
14. week.11: water potential 11 weeks since first measurement (unit: megapascal / missing value: NA)
15. week.12: water potential 12 weeks since first measurement (unit: megapascal / missing value: NA)
16. week.13: water potential 13 weeks since first measurement (unit: megapascal / missing value: NA)
17. week.15: water potential 15 weeks since first measurement (unit: megapascal / missing value: NA)
18. week.17: water potential 17 weeks since first measurement (unit: megapascal / missing value: NA)
19. week.47: water potential 47 weeks since first measurement (unit: megapascal / missing value: NA)
20. week.53: water potential 53 weeks since first measurement (unit: megapascal / missing value: NA)
21. week.94: water potential 94 weeks since first measurement (unit: megapascal / missing value: NA)
22. week.105: water potential 105 weeks since first measurement (unit: megapascal / missing value: NA)

hf348-13: wood anatomy

1. year: year of formation of the ring that contains the band
2. woodid: tree identifier including the position of the microcore on the tree (see POS), when the position is ambiguous
3. image: file name of the analysed image
4. raddistr.band: radial position of the band (unit: micrometer / missing value: NA)
5. rraddistr: radial position of the band as percentage of the fully formed ring width (unit: dimensionless / missing value: NA)
6. mrw: mean ring width (unit: micrometer / missing value: NA)
7. la: mean lumen area (unit: micrometerSquared / missing value: NA)
8. tca: mean total cell area (unit: micrometerSquared / missing value: NA)
9. drad: radial lumen diameter (unit: micrometer / missing value: NA)
10. dtan: tangential lumen diameter (unit: micrometer / missing value: NA)
11. cwa: cell-wall area in the band (unit: micrometerSquared / missing value: NA)
12. cwaacc: cumulative cell-wall area (unit: micrometerSquared / missing value: NA)
13. cwtall: mean cell-wall thickness (unit: micrometer / missing value: NA)
14. cwtall.adj: mean cell-wall thickness of all adjacent cell walls (unit: micrometer / missing value: NA)
15. cwttan: tangential cell-wall thickness (unit: micrometer / missing value: NA)
16. cwtrad: radial cell-wall thickness (unit: micrometer / missing value: NA)
17. kh: hydraulic constant (unit: dimensionless / missing value: NA)
18. eww: earlywood width (unit: micrometer / missing value: NA)
19. lww: latewood width (unit: micrometer / missing value: NA)
20. ewlw.band: radial position of the first band identified as latewood (unit: dimensionless / missing value: NA)
21. ewlw.rpos: radial position of the earlywood to latewood boundary in the ring as percent of the fully formed ring (unit: dimensionless / missing value: NA)
22. ewlw.id: whether the wood is earlywood or latewood
    • ew: earlywood
    • lw: latewood
23. yr.rraddistr.band: radial position of the band as a decimal value of the year (unit: number / missing value: NA)
24. raddist.cont: continuous radial position from the first ring (unit: micrometer / missing value: NA)
25. x.label: year of formation for the first sector of each annual ring
26. plot: treatment group
    • T1: control
    • T2: girdled
    • T3: compressed
    • T4: double compressed
27. tree: tree identifier including the position of the microcore on the tree (see pos)
28. pos: height of microcore on stem
    • A: above
    • B: below
    • M: middle

hf348-14: R code density anomaly analysis

• Compression: zip
• Format: R script
• Type: script