uid=HFR,o=lter,dc=ecoinformatics,dc=org
all
public
read
doi:10.6073/pasta/eab86a290cd2c61f3db9c8b14ebcc323
Effect of Prey Availability on Sarracenia Purpurea Stoichiometry at Belvidere Bog, Vermont 2002
Aaron
Ellison
https://orcid.org/0000-0003-4151-6081
Nicholas
Gotelli
https://orcid.org/0000-0002-5409-7456
Amy
Wakefield
Researcher
Sarah
Wittman
Researcher
2023
English
The carnivorous pitcher plant Sarracenia purpurea receives nutrients from both captured prey and atmospheric deposition, making it a good subject for the study of ecological stoichiometry and nutrient limitation. We added prey in a manipulative field experiment and measured nutrient accumulation in pitcher-plant tissue and pitcher liquid, as well as changes in plant morphology, growth, and photosynthetic rate. Prey addition had no effect on traditional measures of nutrient limitation (leaf morphology, growth, or photosynthetic rate). However, stoichiometric measures of nutrient limitation were affected, as the concentration of both N and P in the leaf tissue increased with the addition of prey. Pitcher fluid pH and nitrate concentration did not vary among treatments, although dissolved oxygen levels decreased and ammonia levels increased with prey addition. Ratios of N:P, N:K, and K:P in pitcher-plant tissues suggest that prey additions shifted these carnivorous plants from P limitation under ambient conditions to N limitation with the addition of prey.
carnivorous plants
leaves
nitrogen
nutrients
phosphorus
photosynthesis
LTER controlled vocabulary
inorganic nutrients
organic matter
primary production
LTER core area
Harvard Forest
HFR
LTER
USA
HFR default
This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.
Creative Commons Zero v1.0 Universal
https://spdx.org/licenses/CC0-1.0.html
CC0-1.0
https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf328
Belvidere Bog (Belvidere VT). Coordinates based on WGS84 datum.
-72.657805
-72.657805
+44.751152
+44.751152
398
398
meter
2002
2002
genus
Sarracenia
species
purpurea
northern pitcher plant
complete
Information Manager
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
hf-im@lists.fas.harvard.edu
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
(978) 724-3595
https://harvardforest.fas.harvard.edu
Prey additions
In May 2002 we haphazardly chose 200 nonflowering adult plants (rosettes ≥10 cm) in the open area of the bog. A distance of at least 50 cm separated all experimental plants. Each plant was randomly assigned to one of eight treatment levels (n = 25 plants per treatment), corresponding to 0-7 frozen houseflies (mean dry mass = 1 mg; 103 mg N/g; 7.6 mg P/g; 9.2 mg K/g; 1.7 mg Ca/g; 1.4 mg Mg/g) fed twice weekly to each pitcher throughout the summer. Flies were added only to pitchers produced during the 2002 growing season; prey addition began when the first new pitchers were produced (24 June 2002) and continued until 12 September 2002. We added distilled water as necessary to maintain pitcher fluid at half of the leaf volume (5–10 mL). Pitchers on each plant were numbered sequentially in order of opening date (Leaf I, Leaf II, Leaf III, etc.) and each leaf was fed until they were harvested for analysis.
Field sample collection and processing
At approximately two-week intervals (10 and 29 July, 13 August, 2 and 15 September), all fed pitchers were harvested from each of five randomly chosen plants from each treatment group. Immediately before each harvest, dissolved oxygen content of the pitcher fluid (milligrams per liter and percentage saturation) of each leaf designated for that harvest was measured in situ using a YSI-58 dissolved oxygen meter (YSI instruments, Yellow Springs, Ohio, USA). Each pitcher was then cut off at the base; pitcher fluid was poured into sterile plastic tubes (50-mL centrifuge tubes) for subsequent analysis and the pitcher itself placed into a Ziploc plastic bag. All harvested material was kept cool and returned to the laboratory at the University of Vermont for processing within 4 h. In the laboratory, the pitcher fluid was filtered using a Millipore Sterifil Aseptic system. The pH of the filtrate was measured with an Orion Model 290A portable pH/ISE meter, and nitrogen (both NO3-N and NH4-N) and phosphorus (PO4-P) concentrations were determined spectrophotometrically using standard methods.
Morphometrics and tissue nutrient content
The length, mouth diameter, and maximum width (±1 mm) of each harvested pitcher or phyllode was measured, along with the width of its wing (keel). All leaves were dried at 70 °C for 3–5 days, and individually weighed and ground for nutrient analysis at the University of Vermont Agricultural and Environmental Testing Laboratory (Burlington, Vermont, USA).
Photosynthesis
On 15 August 2002 we measured maximum photosynthetic rates of the Leaf I on two randomly selected plants from each treatment level. These plants were all harvested at the fourth harvest (2 September). Photosynthesis measurements were made in full sun, between 09:00 and 14:00 hours, using a LI-COR 6200 photosynthesis system and custom built 4-L chamber. During all measurements, ambient solar radiation exceeded the saturating photosynthetic photon flux density of Sarracenia (PPFD > 800 mmol·m-2·s-1).
Harvard Forest Long-Term Ecological Research
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
(978) 724-3595
https://harvardforest.fas.harvard.edu
https://ror.org/059cpzx98
pointOfContact
The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts.
National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210.
hf328-01-sapu-prey-additions-2002.csv
prey additions
hf328-01-sapu-prey-additions-2002.csv
20686
9345cff725be9d15af6cb48a154eb4ff
1
\r\n
column
,
https://harvardforest.fas.harvard.edu/data/p32/hf328/hf328-01-sapu-prey-additions-2002.csv
plantnum
individual plant number
individual plant number
NA
missing value
fliesperwk
number of flies added each week
number
1
whole
NA
missing value
harvest
harvest date
1
10 July 2002
2
29 July 2002
3
13 August 2002
4
2 September 2002
5
15 September 2002
NA
missing value
do.mg.l
dissolved oxygen concentration
milligramsPerLiter
0.01
real
NA
missing value
do.pct
dissolved oxygen (percent saturation)
dimensionless
0.1
real
NA
missing value
halfvol
half of the total volume of the pitcher
milliliter
0.1
real
NA
missing value
lflen
leaf length, measured from the base of the pitcher to the top of the
hood
millimeter
0.01
real
NA
missing value
lfwid
maximum leaf width, measured from the midrib and including the
keel
millimeter
0.01
real
NA
missing value
keelwid
maximum width of the keel, from its outer edge to the edge of the pitcher
tube
millimeter
0.01
real
NA
missing value
diam1
larger diameter of the elliptical pitcher mouth
millimeter
0.01
real
NA
missing value
diam2
smaller diameter of the elliptical pitcher mouth, measured perpendicular
to diam1
millimeter
0.01
real
NA
missing value
leafarea
leaf area of the flattened pitcher
squareCentimeters
0.01
real
NA
missing value
leafmass
dry mass of the pitcher
gram
0.01
real
NA
missing value
ph
pH of the pitcher fluid
dimensionless
0.01
real
NA
missing value
no3
concentration of nitrate-N in the pitcher fluid
milligramsPerLiter
0.01
real
NA
missing value
nh4
concentration of ammonium-N in the pitcher fluid
milligramsPerLiter
0.01
real
NA
missing value
po4
concentration of phosphate-P in the pitcher fluid
milligramsPerLiter
0.01
real
NA
missing value
pct.n
concentration of nitrogen in dried leaf tissue
(percent)
dimensionless
0.01
real
NA
missing value
ca.ppm
concentration of calcium in dried leaf tissue (ppm)
dimensionless
0.1
real
NA
missing value
p.ppm
concentration of total phosphorus in dried leaf tissue
(ppm)
dimensionless
0.1
real
NA
missing value
k.ppm
concentration of potassium in dried leaf tissue
(ppm)
dimensionless
0.1
real
NA
missing value
mg.ppm
concentration of magnesium in dried leaf tissue
(ppm)
dimensionless
0.1
real
NA
missing value
197
community
short-term measurement
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