uid=HFR,o=lter,dc=ecoinformatics,dc=org
all
public
read
doi:10.6073/pasta/4b52a066841ed1ea20346b4643f5e6b5
Effects of Prey Availability on Sarracenia Physiology at Harvard Forest 2005
Aaron
Ellison
https://orcid.org/0000-0003-4151-6081
Elizabeth
Farnsworth
Cheryl
Hester
Researcher
2023
English
Allometric relationships exist between maximal mass-based net photosynthetic rates, leaf mass per unit area, and foliar Nitrogen (N) and Phosphorus (P) content, which hold across a diverse spectrum of over 2500 plant species worldwide. Carnivorous plants depart from this spectrum because they dedicate substantial leaf area to capturing prey, from which they derive N and P under very nutrient-limiting situations. We conducted a manipulative feeding experiment to test whether morphological and physiological allometric relationships of carnivorous plants when nutrients are not limiting are more similar to allometric relationships of non-carnivorous plants.
We examined the effects of prey availability on photosynthetic rate (Amass), chlorophyll fluorescence, growth, architecture, and foliar nutrient and chlorophyll content of ten pitcher plant (Sarracenia) species. We tested the hypothesis that increased prey availability would stimulate Amass of one or more leaves, increase photosynthetic N- and P-use efficiencies (PNUEN, PNUEP), increase relative biomass allocation to photosynthetically efficient, non-predatory phyllodes rather than pitchers, increase overall plant biomass, and reduce stress-related chlorophyll fluorescence. This is the first multi-species, controlled feeding experiment using realistic prey treatments, measuring these physiological parameters directly, and elucidating mechanisms of nutrient stoichiometry and allometry in carnivorous plants.
Increased prey availability increased chlorophyll content, Amass and photosystem efficiency (the Fv/Fm ratio) across the 10 Sarracenia species. These increases were most evident in younger leaves, as older leaves rapidly translocated nutrients to newer, growing tissues. Better-fed plants produced a significantly higher proportion of phyllodes than controls. Higher prey availability was associated with lower N:P ratios, and a shift from P- to N-limitation. PNUEP was significantly enhanced by supplementary feeding, whilst PNUEN was not. Overall biomass and root:shoot ratios were unaffected by feedings.
Feeding shifted allometric relationships of P relative to Amass, N, and LMA from outside the third bivariate quartile to within the 50th bivariate percentile of the relationships found for non-carnivorous plants; other allometric relationships were unaffected. Carnivorous plants can plastically shift phosphorus allometry when nutrients are plentiful, but are less flexible in terms of nitrogen and other ecophysiological parameters.
carnivorous plants
food webs
nitrogen
phosphorus
photosynthesis
plant physiology
LTER controlled vocabulary
populations
LTER core area
Harvard Forest
HFR
LTER
USA
HFR default
This dataset is released to the public under Creative Commons CC0 1.0 (No Rights Reserved). Please keep the dataset creators informed of any plans to use the dataset. Consultation with the original investigators is strongly encouraged. Publications and data products that make use of the dataset should include proper acknowledgement.
Creative Commons Zero v1.0 Universal
https://spdx.org/licenses/CC0-1.0.html
CC0-1.0
https://harvardforest.fas.harvard.edu/exist/apps/datasets/showData.html?id=hf109
Harvard Forest Greenhouse. Coordinates based on WGS84 datum.
-72.19
-72.19
+42.53
+42.53
330
330
meter
2005
2005
genus
Sarracenia
species
alabamensis
species
alata
species
flava
species
jonesii
species
leucophylla
species
minor
species
psittacina
species
purpurea
species
rosea
species
rubra
complete
Information Manager
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
hf-im@lists.fas.harvard.edu
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
(978) 724-3595
https://harvardforest.fas.harvard.edu
Two plants of each Sarracenia species were assigned to one of six feeding levels in a regression design ranging from 0 - 0.25g of finely ground wasps per feeding (for small species), 0 - 0.5g (for species of intermediate sizes), and 0 - 1.0g (for large species); N equals120 plants total. Aboveground size and Amass were measured prior to commencing treatments. Plants were fed once/week for 7 weeks.
Harvard Forest Long-Term Ecological Research
Harvard Forest
324 North Main Street
Petersham
MA
01366
USA
(978) 724-3302
(978) 724-3595
https://harvardforest.fas.harvard.edu
https://ror.org/059cpzx98
pointOfContact
The Harvard Forest Long-Term Ecological Research (LTER) program examines ecological dynamics in the New England region resulting from natural disturbances, environmental change, and human impacts.
National Science Foundation LTER grants: DEB-8811764, DEB-9411975, DEB-0080592, DEB-0620443, DEB-1237491, DEB-1832210.
hf109-01-sarracenia.csv
sarrancenia data
hf109-01-sarracenia.csv
20405
15e8f1fcaf087a0efe14e4b97b045531
1
\r\n
column
,
https://harvardforest.fas.harvard.edu/data/p10/hf109/hf109-01-sarracenia.csv
species
species of pitcher plant used (all species epithets are in genus Sarracenia)
species of pitcher plant used (all species epithets are in genus Sarracenia)
feedlevel
grams of ground hymenoptera fed per week
gram
0.00001
real
NA
missing value
plant.num
number assigned to each plant
number assigned to each plant
fv.fm.lf1
Fv/Fm ratio calculated from dark-adapted chlorophyll fluorescence performed on the first leaf produced by all plants (for which these leaves were available in August 2005)
dimensionless
0.001
real
NA
missing value
fv.fm.lf2
Fv/Fm ratio calculated from dark-adapted chlorophyll fluorescence performed on the second leaf produced by all plants (for which these leaves were available in August 2005)
dimensionless
0.001
real
NA
missing value
totmass
total above- and below-ground plant biomass
gram
0.001
real
NA
missing value
rt.sht
Root:Shoot ratio calculated from aboveground and belowground biomass
dimensionless
0.001
real
NA
missing value
mass.lf
mass of youngest fully-expanded leaf produced by the plant
gram
0.001
real
NA
missing value
area.lf
area of youngest fully-expanded leaf produced by the plant
squareCentimeters
0.1
real
NA
missing value
sla
specific Leaf Area (cm2/g) of youngest fully-expanded leaf produced by the plant
centimeterSquaredPerGram
0.001
real
NA
missing value
chlorophyll
chlorophyll a + b content (mg/g) of youngest fully-expanded leaf produced by the plant
milligramPerGram
0.001
real
NA
missing value
np.ratio
ratio of Nitrogen content to Phosphorus content of youngest leaf produced by plant
dimensionless
0.001
real
NA
missing value
nk.ratio
ratio of Nitrogen content to Potassium content of youngest leaf produced by plant
dimensionless
0.001
real
NA
missing value
kp.ratio
ratio of Potassium content to Phosphorus content of youngest leaf produced by plant
dimensionless
0.001
real
NA
missing value
n
foliar Nitrogen content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
p
foliar Phosphorus content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
k
foliar Potassium content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
ca
foliar Calcium content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
mg
foliar Magnesium content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
na
foliar Sodium content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
s
foliar Sulfur content (%) of youngest leaf
dimensionless
0.001
real
NA
missing value
lma
Leaf Mass Area (grams/square meter) of youngest leaf
gramsPerSquareMeter
0.001
real
NA
missing value
amass
mass-based light-saturated photosynthetic rate of youngest leaf (units: nmol CO2 • g-1 • s-1)
nanomolesPerGramPerSecond
0.001
real
NA
missing value
pnue.n
Photosynthetic Nitrogen Use Efficiency of youngest leaf (units: mmol CO2 • mol N-1 • s-1)
millimolePerMolePerSecond
0.001
real
NA
missing value
pnue.p
Photosynthetic Phosphorus Use Efficiency of youngest leaf (units: mmol CO2 • mol P-1 • s-1)
millimolePerMolePerSecond
0.001
real
NA
missing value
num.lvs
total number of pitchers and phyllodes produced by each plant
number
1
whole
NA
missing value
num.phylls
number of phyllodes produced by each plant
number
1
whole
NA
missing value
mass.phy
mass of a single, randomly-selected, fully-expanded phyllode (when produced by a plant)
gram
0.001
real
NA
missing value
area.phy
area of the same, randomly-selected, fully-expanded phyllode (when produced by a plant)
squareCentimeters
0.001
real
NA
missing value
lma.phy
Leaf Mass Area of the same, randomly-selected, fully-expanded phyllode (when produced by a plant)
gramsPerSquareMeter
0.001
real
NA
missing value
chloro.phy
Chlorophyll a + b content of the same, randomly-selected, fully-expanded phyllode (when produced by a plant)
milligramPerGram
0.001
real
NA
missing value
amass.phy
mass-based light-saturated photosynthetic rate (units: nmol CO2 • g-1 • s-1) of the same, randomly-selected, fully-expanded phyllode (when produced by a plant)
nanomolesPerGramPerSecond
0.001
real
NA
missing value
120
community
short-term measurement
centimeterSquaredPerGram
milligramPerGram
millimolePerMolePerSecond