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Sodalis ligni Strain 159R Isolated from an Anaerobic Lignin-Degrading Consortium
10.1128/spectrum.02346-21
Gina Chaput
Jacob Ford
Lani DeDiego
Achala Narayanan
Wing Yin Tam
Meghan Whalen
Marcel Huntemann
Alicia Clum
Alex Spunde
Manoj Pillay
Krishnaveni Palaniappan
Neha Varghese
Natalia Mikhailova
I-Min Chen
Dimitrios Stamatis
T. B. K Reddy
Ronan O’Malley
Chris Daum
Nicole Shapiro
Natalia Ivanova
Nikos C. Kyrpides
Tanja Woyke
Tijana Glavina del Rio
Kristen M. DeAngelis
Research Article
Research Article
endosymbionts
aromatic metabolism
lignocellulosic biofuel
anaerobic catabolic pathways
aromatic compounds
American Society for Microbiology
Chaput et al.
Copyright © 2022 Chaput et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
20220517
2022
ABSTRACT
Novel bacterial isolates with the capabilities of lignin depolymerization, catabolism, or both, could be pertinent to lignocellulosic biofuel applications. In this study, we aimed to identify anaerobic bacteria that could address the economic challenges faced with microbial-mediated biotechnologies, such as the need for aeration and mixing. Using a consortium seeded from temperate forest soil and enriched under anoxic conditions with organosolv lignin as the sole carbon source, we successfully isolated a novel bacterium, designated 159R. Based on the 16S rRNA gene, the isolate belongs to the genus Sodalis in the family Bruguierivoracaceae. Whole-genome sequencing revealed a genome size of 6.38 Mbp and a GC content of 55 mol%. To resolve the phylogenetic position of 159R, its phylogeny was reconstructed using (i) 16S rRNA genes of its closest relatives, (ii) multilocus sequence analysis (MLSA) of 100 genes, (iii) 49 clusters of orthologous groups (COG) domains, and (iv) 400 conserved proteins. Isolate 159R was closely related to the deadwood associated Sodalis guild rather than the tsetse fly and other insect endosymbiont guilds. Estimated genome-sequence-based digital DNA-DNA hybridization (dDDH), genome percentage of conserved proteins (POCP), and an alignment analysis between 159R and the Sodalis clade species further supported that isolate 159R was part of the Sodalis genus and a strain of Sodalis ligni. We proposed the name Sodalis ligni str. 159R (=DSM 110549 = ATCC TSD-177).
IMPORTANCE Currently, in the paper industry, paper mill pulping relies on unsustainable and costly processes to remove lignin from lignocellulosic material. A greener approach is biopulping, which uses microbes and their enzymes to break down lignin. However, there are limitations to biopulping that prevent it from outcompeting other pulping processes, such as requiring constant aeration and mixing. Anaerobic bacteria are a promising alternative source for consolidated depolymerization of lignin and its conversion to valuable by-products. We presented Sodalis ligni str. 159R and its characteristics as another example of potential mechanisms that can be developed for lignocellulosic applications.
20211205
20220419
2165-0497
Adobe LiveCycle PDF Generator; modified using iText® 5.5.13.2 ©2000-2020 iText Group NV (AGPL-version)2022-07-21T05:40:17-07:00
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